Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

Search Within Results

166 – 180 of 3,503 results

166

Promotions Available

Thermo Scientific Chemicals Bicine, 99+%, for biochemistry

CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: N, N-Bis(2-hydroxyethyl)glycine,Diethylolglycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: [Na+].OCCN(CCO)CC([O-])=O

Pricing & Availability
Specifications

PubChem CID	8761
CAS	150-25-4
Molecular Weight (g/mol)	185.16
ChEBI	CHEBI:40957
MDL Number	MFCD00004295
SMILES	[Na+].OCCN(CCO)CC([O-])=O
Synonym	N, N-Bis(2-hydroxyethyl)glycine,Diethylolglycine
IUPAC Name	2-[bis(2-hydroxyethyl)amino]acetic acid
InChI Key	MFBDBXAVPLFMNJ-UHFFFAOYSA-M
Molecular Formula	C6H12NNaO4

167

Promotions Available

Thermo Scientific Chemicals ADA, 99%, for biochemistry

CAS: 26239-55-4 Molecular Formula: C6H10N2O5 Molecular Weight (g/mol): 190.155 MDL Number: MFCD00008031 InChI Key: QZTKDVCDBIDYMD-UHFFFAOYSA-N Synonym: N-(2-Acetamido)iminodiacetic acid,N-(Carbamoylmethyl)iminodiacetic acid PubChem CID: 117765 ChEBI: CHEBI:43960 IUPAC Name: 2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid SMILES: C(C(=O)N)N(CC(=O)O)CC(=O)O

Pricing & Availability
Specifications

PubChem CID	117765
CAS	26239-55-4
Molecular Weight (g/mol)	190.155
ChEBI	CHEBI:43960
MDL Number	MFCD00008031
SMILES	C(C(=O)N)N(CC(=O)O)CC(=O)O
Synonym	N-(2-Acetamido)iminodiacetic acid,N-(Carbamoylmethyl)iminodiacetic acid
IUPAC Name	2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid
InChI Key	QZTKDVCDBIDYMD-UHFFFAOYSA-N
Molecular Formula	C6H10N2O5

168

Promotions Available

Thermo Scientific Chemicals ACES, 99%, extra pure

CAS: 7365-82-4 Molecular Formula: C4H10N2O4S Molecular Weight (g/mol): 182.194 MDL Number: MFCD00008030 InChI Key: DBXNUXBLKRLWFA-UHFFFAOYSA-N Synonym: N-(2-Acetamido)-2-aminoethanesulfonic acid,N-(Carbamoylmethyl)taurine PubChem CID: 81832 ChEBI: CHEBI:39062 IUPAC Name: 2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid SMILES: C(CS(=O)(=O)O)NCC(=O)N

Pricing & Availability
Specifications

PubChem CID	81832
CAS	7365-82-4
Molecular Weight (g/mol)	182.194
ChEBI	CHEBI:39062
MDL Number	MFCD00008030
SMILES	C(CS(=O)(=O)O)NCC(=O)N
Synonym	N-(2-Acetamido)-2-aminoethanesulfonic acid,N-(Carbamoylmethyl)taurine
IUPAC Name	2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid
InChI Key	DBXNUXBLKRLWFA-UHFFFAOYSA-N
Molecular Formula	C4H10N2O4S

169

Thermo Scientific Chemicals MES-SDS running buffer (20X)

Pricing & Availability
Specifications

Physical Form	Liquid
Recommended Storage	Ambient temperatures
Concentration	20X

170

Promotions Available

Thermo Scientific Chemicals HEPES, 99%, for biochemistry

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid PubChem CID: 23831 ChEBI: CHEBI:42334 SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
Synonym	2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

171

Thermo Scientific Chemicals TBE (5X), TRIS + borate + EDTA

Pricing & Availability
Specifications

MDL Number	MFCD00236358
Packaging	Plastic bottle
Physical Form	Liquid
Chemical Name or Material	TBE (5X)
TSCA	Yes
Recommended Storage	Ambient temperatures

172

Thermo Scientific Chemicals TRIS, 0.5M buffer soln., pH 8.8

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

173

Thermo Scientific Chemicals Phosphate-buffered saline (DPBS, 10X), Dulbecco's formula

Used as a buffer system to maintain cell culture media in the physiological pH range. In combination with glucose, it provides the principal energy sources of cell metabolism. It is involved to irrigate, wash and dilute mammalian cells.

Pricing & Availability
Specifications

Packaging	Plastic bottle
Physical Form	Liquid
Recommended Storage	Ambient temperatures

174

Thermo Scientific Chemicals Tris(hydroxymethyl)aminomethane hydrochloride, Molecular Biology Grade, 99+%

CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 MDL Number: MFCD00012590 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N Synonym: TRIS hydrochloride; 2-Amino-2-hydroxymethyl-1,3-propanediol hydrochloride PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

Pricing & Availability
Specifications

PubChem CID	93573
CAS	1185-53-1
Molecular Weight (g/mol)	157.594
MDL Number	MFCD00012590
SMILES	C(C(CO)(CO)N)O.Cl
Synonym	TRIS hydrochloride; 2-Amino-2-hydroxymethyl-1,3-propanediol hydrochloride
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI Key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
Molecular Formula	C4H12ClNO3

175

Thermo Scientific Chemicals MES-buffered saline (5X), pH 6.5

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate SMILES: O.OS(=O)(=O)CCN1CCOCC1

Pricing & Availability
Specifications

PubChem CID	16218417
CAS	145224-94-8
Molecular Weight (g/mol)	213.25
MDL Number	MFCD00149409
SMILES	O.OS(=O)(=O)CCN1CCOCC1
IUPAC Name	2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate
InChI Key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

176

HEPES, 1M Solution, pH 7.3, Molecular Biology Grade, Ultrapure

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

177

Thermo Scientific™ TRIS, 1.0M buffer soln., pH 8.0

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

178

Promotions Available

MOPS, 99%

CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 4-Morpholinepropanesulfonic acid; 3-(4-Morpholinyl)propanesulfonic acid PubChem CID: 70807 ChEBI: CHEBI:44115 SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1

Pricing & Availability
Specifications

PubChem CID	70807
CAS	1132-61-2
Molecular Weight (g/mol)	209.26
ChEBI	CHEBI:44115
MDL Number	MFCD00006183
SMILES	[O-]S(=O)(=O)CCC[NH+]1CCOCC1
Synonym	4-Morpholinepropanesulfonic acid; 3-(4-Morpholinyl)propanesulfonic acid
InChI Key	DVLFYONBTKHTER-UHFFFAOYSA-N
Molecular Formula	C7H15NO4S

179

Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 8.0

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

180

Thermo Scientific Chemicals Glycine, 0.2M buffer soln., pH 3.0

CAS: 56-40-6 Molecular Formula: C2H5NO2 Molecular Weight (g/mol): 75.07 MDL Number: MFCD00008131 InChI Key: DHMQDGOQFOQNFH-UHFFFAOYSA-N IUPAC Name: 2-aminoacetic acid SMILES: NCC(O)=O

Pricing & Availability
Specifications

CAS	56-40-6
Molecular Weight (g/mol)	75.07
MDL Number	MFCD00008131
SMILES	NCC(O)=O
IUPAC Name	2-aminoacetic acid
InChI Key	DHMQDGOQFOQNFH-UHFFFAOYSA-N
Molecular Formula	C2H5NO2

Life Science Buffers

Filtered Search Results

Narrow Results